Rubén Martínez López - visiting Ph.D. student
I am a visiting PhD student from Dr. Benjami Piña’s lab at the Spanish National Research Council Environmental Assessment and Water Research Centre in Barcelona, Spain. Co-hosted by the Mennigen and Trudeau labs, I utilize zebrafish, Danio rerio as model system to study endocrine disrupting chemicals using transcriptomic and metabolomic approaches.
Zeinab Altmieme - M.Sc. student 2017
I am interested in the role of nonapeptides in reproductive behaviour in male cyprinid fish. I use the zebrafish model, Danio rerio, to investigate the involvement of the neuropeptides isotocin and vasotocin reproductive behaviour, the reproductive endocrine axis and spawning success. To this end I use immunohistochemistry approaches and pharmacological approaches to investigate behavioural, molecular and endocrine reproductive endpoints. This work also has implications for ecotoxicology work investigating nonapeptide targets of endocrine disrupting chemicals in teleost fish.
Melissa Allaire-Leung - 4th Year Honours student
My research projects focuses on the determination of metabolic consequences of developmental exposure of Bisphenol-A (BPA), an endocrine disrupting chemical, at environmentally relevant concentrations in the zebrafish model. I am looking to determine whether short-term exposure during a key developmental period elicits acute or long-term effects on the metabolic phenotype in larvae and adult fish. We have deemed it important to use EE2 as a positive control throughout this study, in order to investigate whether metabolic effects of BPA are mediated by estrogenic compounds.
Kenan Touma - 4th Year Honours student
My project, supervised by Dr. Mennigen and Dr. Gilmour, is focused on testing how the somatotropic axis affects metabolic phenotypes in dominant and subordinate rainbow trout (Onchorynchus mykiss). I will use juvenile rainbow trout as a model to determine whether the GH-IGF axis is differentially regulated at the gene expression and protein level between subordinate and dominant rainbow trout. This will allow improve our understanding of the contribution of this important endocrine axis to the metabolic phenotypes observed in rainbow trout.
Dan Kostyniuk - M.Sc. student 2017
I investigate the nutritional regulation and metabolic function of hepatic microRNAs in rainbow trout, Oncorhynchus mykiss. Specifically, I use molecular, biochemical and bioinformatic methods in vivo and in vitro to identify glucose-regulated microRNAs and to determine whether these miRNAs play a role in ‘glucose intolerance’ in this species.
Mais Jubouri - M.Sc. student 2018
In my M.Sc. project co-supervised by Dr. Mennigen and Dr. Weber, I investigate the hypothesis that nutrient interactions play a role in the glucose intolerant phenotype in rainbow trout. Specifically I combine organismal level approaches investigating the role of specific infused AA on glucose fluxes in rainbow trout with cellular signalling and molecular gene expression endpoints in order to integratively determine the role of specific AA on rainbow trout glucose metabolism.
Alexandria Trahan - 4th Year Honours student
I am investigating the acute and long-term metabolic effects of embryonic exposure to the plasticizer DEHP, a known endocrine disrupting chemical, in zebrafish. My thesis focuses on determining whether DEHP acts as a metabolic disruptor acutely in larvae and/or programs developmental trajectories towards an altered metabolic phenotype in adult zebrafish. A secondary aspect of my project is to identify whether possible metabolic effects can be linked to an anti-androgenic mode of action, which I test by using flutamide as a positive anti-androgenic control.
Christine Hum - 4th Year Honours student
My project investigates the metabolic consequences of the fluorinated compound F-53B, a recently discovered aquatic contaminant in Chinese aquatic systems in developing zebrafish. I use a suite of zebrafish larvae metabolic phenotyping assays in conjunction with gene expression approaches to identify potential effects F-53B as well as the fluorinated compounds PFOS and OBS at concentrations corresponding to 10%, 1% and 0.1% of the identified LC50 concentrations of each compound.
Mennigen lab alumni
Wenqin Tu - Visiting Scholar
I am an ecotoxicologist based at the Jiangxi Academy of Sciences, Nanchang with an interest in fluorinated compound toxicity, which I investigate in the zebrafish model using wet-lab and predictive approaches. In the Mennigen lab, I investigate sublethal metabolic effects of the fluorinated compounds F-53, PFOS and OBS, which have been detected in Chinese aquatic environments.
Adina Gotzmann - 4th Year Honours student 2017/2018
I investigate the nutritional (fasting/refeeding and glucose injection) and endocrine (insulin injection) transcriptional regulation of key metabolic genes as well as insulin cell signalling pathways in liver and muscle of the North Pacific spiny dogfish, Squalus suckleyi.
Jessica Zon - 4th Year Honours student 2017/2018
My project co-supervised by Dr. Mennigen and Dr. Trudeau is focused on investigating paternal and maternal mechanisms associated with the intergenerational of altered stress responses in zebrafish offspring derived from parents previously exposed to the pharmaceutical fluoxetine, an aquatic contaminant in urban watersheds. In this project, I am particularly interested in quantifying maternally deposited transcripts involved stress axis programming, as well as transcripts involved in molecular epigenetic pathways.
Lucie Marandel - Visiting Scholar
I am a group leader at INRA NuMeA, where my research group investigates the role of teleost and salmonid gene paralogues in glucose metabolism in rainbow trout. I additionally use targeted epigenetic profiling to investigate potential contributions of DNA methylation and Histone modifications in rainbow trout glucose metabolism and as tool to probe the feasibility of nutritional programming approaches in rainbow trout. A recent development in my research is the determination of sex specific nutritional programming effects in offspring.
Rida Haider - 4th Year Honours student 2017/2018
I investigate the possible role of the nonapeptide isotocin as mediator of reproductive endpoints in response to pheromone signalling in male goldfish, Carassius auratus. I am furthermore interested in investigating the role of social context on the stimulation of the male reproductive axis in the same species.
Majd Haddad - 4th Year Honours student 2017/2018
My project co-supervised by Dr. Mennigen and Dr. Trudeau is focused on investigating paternal and maternal inheritance of stress response in zebrafish exposed to the pharmaceutical fluoxetine, an aquatic contaminant in urban watersheds. In this project, I am developing assays for microRNA quantification in zebrafish egg and sperm cells to investigate their possible involvement in maternal or paternal inheritance of contaminant induced alterations in the stress response.